Composite

Part:BBa_K4410007:Design

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZBIOX   (2022-09-21)


J23100-argJ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
    Illegal PstI site found at 1004
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 769
    Illegal PstI site found at 319
    Illegal PstI site found at 1004
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
    Illegal PstI site found at 1004
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
    Illegal PstI site found at 1004
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To maximize the arginine production and avoid repressive effect, we insert ArgJ gene into pGLO plasmid which Including j23100 promoter, ribosome binding site and argj gene.Then through electrotransformation between pGLO and EcN1917-PKD46, we successfully insert the ArgJ fragment into EcN1917.


Source

The KanR has been deposited in GenBank under the accession number CGL_RS06970.

References